Chromatography Forum

Hi,

Does anyone have any idea if the GC split ratio is proven to be linearly related to the peak response? i.e. If I double the split ratio, the peak response that I should be getting would be halved. I tried doing a search and read that this may only work for split ratios between 25:1 to 200:1. Not sure if this is true because I can't find proper literature to prove this.

Thank you!

I cannot cite any literature, but the orthodox position is that the relationship of peak area to split ratio is not linear enough to be analytically useful.

This is probably one of those situations where a simple experiment in your own lab would be worth a ton of literature; in the words of a previous forum member (Hans Muller) "Don't think, measure".

Peter

We went from splitless injection to a 5:1 split injection and the responses went up, so it is not just a matter of the amount of gas going to the column versus the amount going out the split vent.

I did however do a test this week where I injected 2ul at 20:1 and 4ul at 40:1 and got nearly identical responses.

It is not a linear relationship. You have to test it yourself on your instrument with your method.