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analysis of sugar by LCMS

http://www.chromforum.org/viewtopic.php?t=3413

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analysis of sugar by LCMS

Posted: Mon Feb 13, 2006 10:32 pm
by jmathew
Analysis of sugar is done by HPLC using RI detector or PAD detector.
is there any method using LCMS?

John

Posted: Tue Feb 14, 2006 4:54 pm
by tom jupille
http://www.google.com/search?hl=en&q=gl ... gle+Search

Posted: Wed Feb 15, 2006 4:16 pm
by Noser222
You can get good sensitivity in APCI- mode with chloride adducts. I've done this with an amino column with a mobile phase of 80-85% acetonitrile. You can add methylene chloride or chlorform post-column, but I've added a little to the mobile phase and it worked great.

Re: analysis of sugar by LCMS

Posted: Wed Feb 15, 2006 6:07 pm
by mmadson53
Analysis of sugar is done by HPLC using RI detector or PAD detector.
is there any method using LCMS?

John
What particular types of samples are you interested in? One needs to be a bit more specific. Also what kinds of carbohydrates are you looking for?

Mike
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