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LC/MS Buffer - Can we use TRIS?

http://www.chromforum.org/viewtopic.php?t=3384

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LC/MS Buffer - Can we use TRIS?

Posted: Wed Feb 08, 2006 7:03 pm
by MPaciol
Hello All,

We have developed an LC/MS compatible method...or so I thought. We are using a TRIS Buffer pH 8.0 and now I'm not so sure we can use it with LC/MS. Would someone please comment on this or suggest another buffer that can be used at pH 8?

MPaciol

Posted: Thu Feb 09, 2006 7:59 pm
by Mark Tracy
Ammonium bicarbonate. Not very stable, but MS compatible.

Posted: Thu Feb 09, 2006 8:45 pm
by Uwe Neue
I myself got a bit disappointed with ammonium bicarbonate at close to neutral pH. pH 8 might still work, but I recoomend to measure the pH every few hours until you are comfortable that it does not change.
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