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- Posts: 4
- Joined: Wed Jul 03, 2013 6:57 am
Background:
I've been using an old Agilent 1100 to fractionate my samples prior to LCMSMS. I measure nucleoside modifications so my samples are comprised of enzymatically hydrolyzed DNA. The final quantification uses both LCMSMS data (for measuring the low abundance modified nucleosides) and LC-UV data (for measuring the high abundance unmodified nucleosides). That is, I measure the fractionated nucleosides an LCMSMS and normalize them to the chromatograms of the four canonical nucleosides from an LC-UV during fractionation.
My problem:
The chromatogram from the LC-UV is a simple four-peak affair with good signal-to-noise and clear baseline separation (using OpenLab software, previously called ChemStation). I almost never have to manually integrate the peaks. However, I cannot figure out how to get the data in an Excel (or CSV) format for all the samples at once. Thus far, I have found the simplest solution to be mass-printing the PDFs (I like to keep hard copies anyway) and manually typing the peak areas into Excel for quantification! I always tell myself I'll figure out a better way but never got around to it.
My question:
How do I obtain a tabularized version of my results that shows the peak areas for all four peaks for all my samples in a given run?
Thanks in advance for your help!