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- Posts: 1
- Joined: Tue Jul 26, 2016 2:17 pm
Dear experts,
I performed a peak purity (and match purity) on a DAD HPLC using Empower 2.0 as software. Injecting 6 replicates of a pure standard and the software automatically calculated Purity Angle (PA) and Threshold (PT). I used PA and Match Angle (MA) of std's replicates as Solvent Angle (with Threshold Noise+Solvent) for my samples. Result are: Purity Angle < Threshold (ok) but MA > Match Threshold. I've used all the technical advise for performing a better work (resolution, AU < 1, noise range...). I've seen also match result and the differences between std and sample are minimum. I can't explain this problem...
I performed a peak purity (and match purity) on a DAD HPLC using Empower 2.0 as software. Injecting 6 replicates of a pure standard and the software automatically calculated Purity Angle (PA) and Threshold (PT). I used PA and Match Angle (MA) of std's replicates as Solvent Angle (with Threshold Noise+Solvent) for my samples. Result are: Purity Angle < Threshold (ok) but MA > Match Threshold. I've used all the technical advise for performing a better work (resolution, AU < 1, noise range...). I've seen also match result and the differences between std and sample are minimum. I can't explain this problem...
