Chromatography Forum

Hi

Can somebody suggests the various techniques that can be used to analyse the deaminated peptide.

Thanks

Dear Pawan,

We have few application and columns for peptide analysis (small peptides and amino acids):
http://www.sielc.com/index.html

We are also located in Illinois you can contact us directly an we will try to find solution for your separation problem (our office is located in Prospect Heights, IL). You can call or visit us.

Regards,

Vlad

I assume you meant to write "deamidated." The conversion of glutamine to glutamate or asparagine to aspartate changes the net charge. Therefore the most common methods are weak anion exchange or weak cation exchange. The variant will elute early by cation exchange or late by anion exchange. If the peptide is not too big, typical reversed-phase conditions for peptide analysis (H2O/MeCN/TFA gradient) will also do the job. Of course, you can combine the separation modes.

Thank you for your suggestions.
Is there any way to confirm the absence of deamidated peptide in the API. I donot have any deamidated peptide standard available with me.