Chromatography Forum

HI, Everybody.
This is Vijay from India, I have a problem with my Waters HPLC detector, it shows "Unable to detect 656nm low energy. I am having a waters system with the follwoing specification......
1) Dual wavelenght detector.
2) 1515 model waters Pump.
3) Breeze software.

I am havinig an inclination on UV bulb. If some body has experienced same problem let me know what thas to be done.

Thank in advance[/b]

I am not familiar with this detector, but did you check its wavelenght limit?

Usually, single lamp detectors with Deuterium lamps have a wavelnght limit up to 600 nm.

Dual lamp detectors (Deuterium + Tungsten) could go up to 800 nm.

Eventually your system alows 656 nm set but the lamp presents very low energy at that level...

Vijay


When the detector starts up, it looks for the 656nm spike that is in the D2 spectrum. If it can not find that spike it generates that error message. You either need a new lamp or your flow cell is blocked or dirty.

when lamp intensity reduses , it finds difficult to get start D2 lamp at 656 nm .Needs to replace new lamp or even if you switch off and on the detector it wont ignite and shows error.

It may be not because of flow cell blockness, consider this.

can you remove the cell and try again? This allows you to locate the problem, i.e. lamp or dirty flow cell.