Chromatography Forum

how we select a column for particular compound and would we go a compound non ionic neutal ) on reversed phase with ion exchange

how we select a column for particular compound and would we go a compound non ionic neutal ) on reversed phase with ion exchange

For neutral (non-ionic) compounds RP columns are preferred. For ionic compounds you can use a ion pairing reagent to work with RP columns. If not possible ion exchange columns provide an option.

Regards,

Amaryl.

You will find a lot of this information in my book on "HPLC Columns" available from Wiley-VCH. You can obtain it from your Waters catalogue, if you do not have access to a book store that carries or to amazon.com.

You appear to be working in the pharmaceutical industry. Under these conditions, the selection of the correct column should be determined by the reproducibility of the column. Publications by Guiochon in J. Chromatogr. have looked at this for several commercial packings.

Check this step by step method development guide (based on the structure of your compound):

http://www.sielc.com/MethodDevelopment_ ... dType.html

It will ask you bunch of questions about your compound and show you some examples

Another one is based on pKa values and detection technique:

http://www.sielc.com/MethodDevelopment_Guide.html


Here is comparison for RP, ion-exchange and mixed mode approaches:

http://www.sielc.com/Technology_2D_Properties.html


Regards,

Vlad

Hi,

There is no such thing like "a column for a particular compound". Chromatographic resolution is influenced by the analyte, the column and the chromatographic conditions. So, in some cases any column will do and for others you will have to search a little longer.
To get a first impression, search the column manufacturers catalog for application notes. probably you won't find your substance, so you will have to generalise.

Alex

Thank you SIELC_Tech for the website references. I have looked for something like this on the web for years.