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HPLC with DMSO as solvent
Posted: Fri Jan 20, 2006 1:20 am
by b_daniel
Is it possible to use HPLC to analyze the concentration of small molecules dissolved in DMSO? If not, is there a suitable method?
Posted: Fri Jan 20, 2006 2:57 am
by james little
We use DMF and DMSO for dissolving samples for LC-MS analyses.
As long as you keep the injection volume low, 5-10 ul's, causes no serious problems and still gives decent peak shapes.
Re: HPLC with DMSO as solvent
Posted: Fri Jan 20, 2006 2:42 pm
by amaryl
Is it possible to use HPLC to analyze the concentration of small molecules dissolved in DMSO? If not, is there a suitable method?
DMSO is used as surfactant to solubilise sample in HPLC. Being a strong solvent should be used in less amount.
Regards,
Amaryl.
Posted: Mon Jan 23, 2006 12:45 am
by syx
We have used DMSO (water-free) as diluent for a substance that very sensitive to water. The sample is injected in less than 20 uL and found no significant peak distortion.
DMSO usage
Posted: Sat Feb 04, 2006 1:07 pm
by avitan
syx wrote:We have used DMSO (water-free) as diluent for a substance that very sensitive to water. The sample is injected in less than 20 uL and found no significant peak distortion.
Was it with Normal-phase or Reversed-phase mode?
Posted: Mon Feb 06, 2006 1:52 am
by syx
RP-LC. The mobile phase contains buffer solution. However, the aqueous content of the mobile phase did not degrade the active substance, because the analysis time is not more than 15 minutes.