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RP-Amide HPLC column stability
Posted: Fri Jul 08, 2016 3:10 pm
by skp
Does anyone have experience with using a reversed phase Amide column? It seems that it takes a long time for the column to come ready for use - excessively long time required for conditioning, is this typical for this phase or should I be looking for something else. The mobile phase is 65:35 ACN:Water with a gradient to 100% ACN.
Re: RP-Amide HPLC column stability
Posted: Sat Jul 30, 2016 3:46 am
by mattmullaney
Nope, I've not observed anything such you have experienced with either the RP-Amide or its superficially-porous cousin, the "Express" version, nor with the Bonus-RP from Agilent. Is your injection solvent "weaker" in elution strength than 65:35 ACN/water?
Re: RP-Amide HPLC column stability
Posted: Mon Aug 01, 2016 5:46 am
by Gerhard Kratz
I agree with Matt.