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- Posts: 8
- Joined: Sat Jul 02, 2016 2:55 am
Hi there,
first I have to apologize. Mass spectrometry is not my area of expertise. However I´m trying to learn.
So we have an LTQ Orbitrap that we are using for measuring peptides of a protein coming from a C18 column. The system works fine.
What I need is a way of identifying the peptides. And this is where the trouble starts:
I have installed Bioworks 3.3.1 SP1 (I think). I wanted to use it to do a sequest search for the peptides. But if I go to "actions" the menu point "sequest search" is gray and I can´t do anything with it. In the Bioworks configuration under "search engines" I should have Sequest as an entry, but it is not there. I don´t know what to do. The Bioworks manual does not help either.
Does anyone here has an idea how to fix this?
All I want to do is to open the *.file from the LTQ, run a sequest search for the peptides and generate an exclusion list that I can give back to the mass spec so that it ignores well populated peptides and also looks for some that are not so much populated.
Hoping for help here.
Thx in advance.
Cheers
first I have to apologize. Mass spectrometry is not my area of expertise. However I´m trying to learn.
So we have an LTQ Orbitrap that we are using for measuring peptides of a protein coming from a C18 column. The system works fine.
What I need is a way of identifying the peptides. And this is where the trouble starts:
I have installed Bioworks 3.3.1 SP1 (I think). I wanted to use it to do a sequest search for the peptides. But if I go to "actions" the menu point "sequest search" is gray and I can´t do anything with it. In the Bioworks configuration under "search engines" I should have Sequest as an entry, but it is not there. I don´t know what to do. The Bioworks manual does not help either.
Does anyone here has an idea how to fix this?
All I want to do is to open the *.file from the LTQ, run a sequest search for the peptides and generate an exclusion list that I can give back to the mass spec so that it ignores well populated peptides and also looks for some that are not so much populated.
Hoping for help here.
Thx in advance.
Cheers
