How to operate a Waters Nano Acquity without Sample Manager?
Posted: Sat Jul 02, 2016 3:13 am
Hey there,
I have recently got a Nano Acquity system (Sample manager, Binary Solvent Pump and Auxilliary Solvent Pump) that I want to use for some special purpose. It will operate a Pepsin column for online digestion, followed by trapping of the peptides and then separation of the peptides followed by injection into a Mass Spectrometer.
For this purpose I don´t need the Sample manager - it is too slow for me and I need my samples to be cooled once they are in the column. The whole system is already set up for manual injection.
I would like to use the Acquity system without the sample manager. Is that possible? I can kick out the sample manager from the system configuration (Acquity console) but I can not write a method that works without that this thing is required to be connected. It should not be too hard to just get the pumps working - one of them needs to be in isocratic mode and the other needs to run a gradient when I want it.
Unfortunately the Sample manager has some issues and does not work correctly but I don´t want to spend thousands of dollars on this thing as I do not need it (and never will...) for my setup.
Maybe somebody here tried a similar thing and can help me with that issue?
Thanks in advance!
I have recently got a Nano Acquity system (Sample manager, Binary Solvent Pump and Auxilliary Solvent Pump) that I want to use for some special purpose. It will operate a Pepsin column for online digestion, followed by trapping of the peptides and then separation of the peptides followed by injection into a Mass Spectrometer.
For this purpose I don´t need the Sample manager - it is too slow for me and I need my samples to be cooled once they are in the column. The whole system is already set up for manual injection.
I would like to use the Acquity system without the sample manager. Is that possible? I can kick out the sample manager from the system configuration (Acquity console) but I can not write a method that works without that this thing is required to be connected. It should not be too hard to just get the pumps working - one of them needs to be in isocratic mode and the other needs to run a gradient when I want it.
Unfortunately the Sample manager has some issues and does not work correctly but I don´t want to spend thousands of dollars on this thing as I do not need it (and never will...) for my setup.
Maybe somebody here tried a similar thing and can help me with that issue?
Thanks in advance!