Chromatography Forum

Hello,

I'm currentlly running a method for the quantitative determination of alendronate using a FLD detector. It seems that the peak of alendronate merges with a subsequent peak whose intensity remains constant. In most cases this merging results in a shoulder or extensive tailing of alendronate. Is there a way i can perform a split between these two peaks and are there any criteria for accepting the integrated in such mannner chromatogram?

Can you give more details on your method? Otherwise, is a bit difficult to give an opinion...

Looking at your method, you might be suffering either column degradation, since your pH is too high for "normal" silica based columns, or alendroate is partially ionized them generating a splitted peak.

Amines are known to create such problems. I would recommend you to try a Phenomenex Gemini C18 or similar column and increase your pH.