need help regarding LC-MS-NMR

[aniket]

hi to all,
i am a necomer to the field of liquid chromatography, i heard from mates about LC-MS-NMR, can anyone tell me some thing about it and its use?
can someone suggest some references? please help?

[Alex Buske]

LC-Ms is the coupling of HPLC with a suitable mass spectrometer, LC-NMR with a especially equipped NMR.
LC-MS is frequently used, LC-NMR less often. For both systems you would need different solvent systems: LC-MS needs volatile buffers, LC-NMR deuterated solvents.
Both systems give complementary information, in some cases structure elucidation is possible. The amount of sample needed is less than with isolation, especially with LC-NMR the column has to be overloaded to the maximum.
Both systems require trained and experienced staff for usable results.

Alex

[Ary]

Hi Aniket

I've co-authored around 20 papers on this technique so I guess I can say I know what's what. Try doing a search on I D Wilson or F Pullen as a starting point. These guys have been at the forefront (amongst others before I upset someone). If you have any specific questions just let me know.

Steve

[aniket]

thank you so much ary, i am intested to know what are bascis of LC-NMR-MS? also i heard that it used for impurity profiling? am i right?
can you please suggest me some references?
thank you
aniket
proud indian

[Kostas Petritis]

Articles from JC Lindon are good for what you ask. He is emphasizing more NMR than MS but it is a good starting point...

[Ary]

http://www.spectroscopyeurope.com/NMR_16_3.pdf

http://www.spectroscopynow.com/coi/cda/ ... ure&chId=5

The two references above give a useful insight as well as providing good bibliogarphies with lots of helpful further reading