amino acids by gc

[upamaniah]

hi,
amino acid by gc.
any suggestions.
vijay

[Rafael Chust]

Hello again!

www.phenomenex.com

EZ:faast AA analysis kit, available for GC-FID, GC-MS and LC-MS.

[Srinivas SK]

Silyl derivatisation, followed by FID or Ion selective MS, depending on the sample matrix and sample concentration.

If it's a biological matrix, like serum, there will be considerable sample prep to be done.

rgds,

S.K. Srinivas

[foren]

Hi all,
I am trying to set up a method using ez faast Protein Hydrolysate kit. Sample preparation variations are much higher than I expected.
When I test Pickering aa reference standarts (each AA 250nmol/ml) after fresh calibration I found that results are varying, ie, some are around 200, some 125.
I don't have much time for troubleshooting until next year .
Any similar experience?

Happy new year to all.

Foren