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need help regarding LC-MS-NMR
Posted: Fri Dec 16, 2005 9:30 am
by aniket
hi to all,
i am a necomer to the field of liquid chromatography, i heard from mates about LC-MS-NMR, can anyone tell me some thing about it and its use?
can someone suggest some references? please help?
Posted: Fri Dec 16, 2005 10:42 am
by Alex Buske
LC-Ms is the coupling of HPLC with a suitable mass spectrometer, LC-NMR with a especially equipped NMR.
LC-MS is frequently used, LC-NMR less often. For both systems you would need different solvent systems: LC-MS needs volatile buffers, LC-NMR deuterated solvents.
Both systems give complementary information, in some cases structure elucidation is possible. The amount of sample needed is less than with isolation, especially with LC-NMR the column has to be overloaded to the maximum.
Both systems require trained and experienced staff for usable results.
Alex
Posted: Fri Dec 16, 2005 3:24 pm
by Ary
Hi Aniket
I've co-authored around 20 papers on this technique so I guess I can say I know what's what. Try doing a search on I D Wilson or F Pullen as a starting point. These guys have been at the forefront (amongst others before I upset someone). If you have any specific questions just let me know.
Steve
Posted: Mon Dec 19, 2005 11:22 am
by aniket
thank you so much ary, i am intested to know what are bascis of LC-NMR-MS? also i heard that it used for impurity profiling? am i right?
can you please suggest me some references?
thank you
aniket
proud indian
Posted: Mon Jan 02, 2006 2:26 am
by Kostas Petritis
Articles from JC Lindon are good for what you ask. He is emphasizing more NMR than MS but it is a good starting point...
Posted: Tue Jan 03, 2006 3:08 pm
by Ary
http://www.spectroscopyeurope.com/NMR_16_3.pdf
http://www.spectroscopynow.com/coi/cda/ ... ure&chId=5
The two references above give a useful insight as well as providing good bibliogarphies with lots of helpful further reading