student needs help! :-)
Posted: Thu Dec 15, 2005 10:41 am
by mibemolle
I need to know how "build up" a good method of hplc analisys of Doxazosin Mesylate in human serum (et/or urine).......
Please help me... (I'm so ignorant.......
)
thanks
Mib
Posted: Thu Dec 15, 2005 4:56 pm
by Uwe Neue
The more difficult part is not the HPLC method, but the sample cleanup.
For the HPLC method, I suggest to run a gradient from 5 to 95% organic on a 5 cm x 4.6 mm 3 micron column over 20 to 30 mL of gradient volume. Initial pH set up with 20 mM acetate buffer on a modern C18 column.
For the sample preparation, I definitely recommend solid-phase extraction. Look at the Waters website for the Oasis applications notebook! It contains detailed information how to do this.