method of methscopolamine, phenylephrine & chlorphenaram

[ym3142]

Hi,

Please help if you are interested in challenging separation.
1) all the following separation need to pass PDA purity test (wave length 200 to 300nm) and Rs >2.3;

2) separated the oxidation degradants from phenylephrine (20mg phenylephrine in 10 mL water containing 1 mL 3% H2O2 is heated over steam bath for 15 min to get the oxidation);

3) the same for CPM as 2) except for 30 min heating for CPM oxidation;

4) separate the impurity peak coming with sample diluent 0.2M KH2PO4 and showing up around CPM

5) the whole method run time < 30 min for separation of the all three actives in the title.

[SIELC_Tech]

Excel,

we will take the challenge, we have bunch of method for cough medications. A lot of these compositions have the same compounds in differnt ratios (ephedrines, acetaminophen, chrlorpheniramine, phenylephrine, doxylamine, etc.).

Here is some links for you:
http://www.sielc.com/application_122.html
http://www.sielc.com/compound_166.html
http://www.sielc.com/compound_116.html

We also have couple more methods which are not on the web yet. I'll send you few files in couple of days.

Regards,

Vlad

[ym3142]

Vlad,

first of all, thank you for the two methods(since 122=166). they are good works but I will only put down my concerns.
before we know how your methods separates the api from their impurity and degradants, for 122/166, 1) it is running gradient without even base line, which we do not like as for quantitation. and 2)i am not sure the Rt for chlor(less hydrophilic , afraid to be long);
for 116, if we make the Rt for phenylephrine >3.5min(double of the void), i afriad 1) the peak shape of acetaminophen makes me think phenylephrine was not separated from its degradants w/ Rs>2.3; 2) also may long Rt for chlor.