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HPLC Column
Posted: Fri Apr 29, 2016 1:10 pm
by Tfes
is it possible to acetone for reverse phase HPLC?
Re: HPLC Column
Posted: Fri Apr 29, 2016 1:53 pm
by HPLCaddict
Yes, basically it is possible to use acetone (and it actually has a quite high elution power, higher as acetonitrile) but the UV-cutoff is pretty high. I don't remember exactly but I think it's higher than 300 nm, so working in the usual UV range is rather impossible.
Re: HPLC Column
Posted: Fri Apr 29, 2016 6:51 pm
by itspip
If you plan on using a UV-Vis/DAD for detection chances are you're out of luck. If you plan on using refractive index, it should work.
Re: HPLC Column
Posted: Mon May 02, 2016 12:20 pm
by Gerhard Kratz
And if your sealings will withstand Acetone yes!
Re: HPLC Column
Posted: Tue May 03, 2016 2:02 pm
by carlo.annaratone
I quote Gerhard, check the compatibility of your seals and tubing