Chromatography Forum

My company inherited a very fancy sonicator (specifically the Q800R2 from QSonica http://www.sonicator.com/19-q800r2-sonicator.html) that we are thinking of bringing into our workflow.

The vast majority of our samples we process are small molecules in plasma. For now, sample prep has been very simple: mixing into acetonitrile, centrifuging, and collecting the supernatant.

Are there any advantages to using the sonicator? Lower LLOQ? Better for other types of fluids or tissue?

Thanks in advance,

Ken

Welcome to the forum.

Rule number one; if it ain't broke, don't fix it.

Sonication only helps if you have immiscible phases.

Peter

People often sonicate after adding acetonitrile and before centrifugation to help release drugs from plasma proteins. You might make a quick comparison with and without sonication.