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Benzalkonium Chloride Method
Posted: Wed Nov 30, 2005 5:05 pm
by msloane
This is a pretty simple post, i hope. I'm looking for a Benzalkoium lc uv method that uses something other than a cyano or SCX column. Any help would be great.
Posted: Wed Nov 30, 2005 5:47 pm
by Consumer Products Guy
Look for Chrompack application note #99, uses ion paring on a C18 column.
Posted: Wed Nov 30, 2005 5:52 pm
by SIELC_Tech
We have a method for similar compound cetylpyridinium chloride with perfect symmetry and retention control. Check the following link, it also shows retention on C18 column. Our column is a mixed mode column with combination of reverse phase and ion-exchange groups.
http://www.sielc.com/compound_113.html
Compounds like benzalkonium tends to tail a lt due to residual silanol interaction, evan end-capped columns show tailing (in our comparisson it was leading brand C18 end capped column).
Contact us if you have questions.
regards,
Vlad
Posted: Wed Nov 30, 2005 6:32 pm
by rhaefe
Hamilton Application Note #280. Uses a PRP-1 reversed phase column in straight RP mode.
Best regards,
Robert Haefele
rhaefele@hamiltoncompany.com
Posted: Wed Nov 30, 2005 7:01 pm
by SIELC_Tech
Dear Robert,
Can you provide a link to the method. I tried to find it on the website but did not see it.
you can email it to
vlad.orlovsky@sielc.com
I want to compare it to our method (peak shape for quaternary amines)
regards,
Vlad
Posted: Wed Nov 30, 2005 7:16 pm
by rhaefe
http://www.hamiltoncomp.com/cgi-shl/pro ... applist280
Peak shape is actually not that great. Some improvement might be possible by running at elevated T and by using a 3 or 5um PRP-1 column. The application note is pretty old (>15yrs?) which explains the 10um particle size.
best regards,
Robert Haefele
rhaefele@hamiltoncompany.com
Posted: Sat Dec 03, 2005 12:15 am
by Mark Tracy
You could also try the Acclaim Surfactant column. Featured on the homepage at
www.dionex.com
amino phase
Posted: Mon Dec 05, 2005 4:38 pm
by Noser222
I have used amino phase for LC-MS of these compounds.