Chromatography Forum

Im running an Escitalopram sample via HPLC. However, the standard being used as per USP is the optical isomer "Citalopram". The column to be used is L1 4.6 x 100 mm, 3micron.

Question.

With the above given column specification, would I expect almost the same retention time if I'm going to inject both Optical isomers?

Since you're doing simple reversed-phase chromatography, Escitalopram and (R-)Citalopram won't have almost the same retention time. They will have exactly the same retention time:).
You'd need a chiral column to produce different retention times for these two.