Advertisement

How to operate a Waters Nano Acquity without Sample Manager?

Discussions about HPLC, CE, TLC, SFC, and other "liquid phase" separation techniques.

2 posts Page 1 of 1
Hey there,
I have recently got a Nano Acquity system (Sample manager, Binary Solvent Pump and Auxilliary Solvent Pump) that I want to use for some special purpose. It will operate a Pepsin column for online digestion, followed by trapping of the peptides and then separation of the peptides followed by injection into a Mass Spectrometer.
For this purpose I don´t need the Sample manager - it is too slow for me and I need my samples to be cooled once they are in the column. The whole system is already set up for manual injection.
I would like to use the Acquity system without the sample manager. Is that possible? I can kick out the sample manager from the system configuration (Acquity console) but I can not write a method that works without that this thing is required to be connected. It should not be too hard to just get the pumps working - one of them needs to be in isocratic mode and the other needs to run a gradient when I want it.
Unfortunately the Sample manager has some issues and does not work correctly but I don´t want to spend thousands of dollars on this thing as I do not need it (and never will...) for my setup.
Maybe somebody here tried a similar thing and can help me with that issue?
Thanks in advance!
Just an update. It is not possible to operate the acquity system without the sample manager. This is a bitter lesson and I wonder why industry designs such a stupid thing (profit as always I guess). Anyway we were able to get the UPLC up and running and now we have the sample manager inject air.
Cheers
2 posts Page 1 of 1

Who is online

In total there are 20 users online :: 1 registered, 0 hidden and 19 guests (based on users active over the past 5 minutes)
Most users ever online was 4374 on Fri Oct 03, 2025 12:41 am

Users browsing this forum: Majestic-12 [Bot] and 19 guests

Latest Blog Posts from Separation Science

Separation Science offers free learning from the experts covering methods, applications, webinars, eSeminars, videos, tutorials for users of liquid chromatography, gas chromatography, mass spectrometry, sample preparation and related analytical techniques.

Subscribe to our eNewsletter with daily, weekly or monthly updates: Food & Beverage, Environmental, (Bio)Pharmaceutical, Bioclinical, Liquid Chromatography, Gas Chromatography and Mass Spectrometry.

Liquid Chromatography

Gas Chromatography

Mass Spectrometry