Chromatography Forum

Dear all,

Recently, i try to plot a calibration curve using the feature stated in Agilent Chemstation and I faced several problems, may be some of you have came across this problem too...please help.

I carry out the test with "Bracketed Sequence" and untill the GC has completed the sequence, I dun know where to look for my report...help

What version of chemstation are you using?

You might as well shoot yourself as try to get anything useful out of the Chemstation calibration curve functionality, in my humble opinion. There are certainly many people who use it successfully every day, and I myself have used it in the past to identify peaks by retention time. However, the poor documentation of previous versions (everything up to A.10.00), the awkward resitrictions on when standards must be run in the sequence and how the calibration is calculated, and above all, the bugs (aka "undocumented features") long ago drove me to use MS Excel for all quantitiative calculations of ChemStation results. In harsh contrast to ChemStation macro language, MS Visual Basic for Applications is a well-documented, versatile programming language that can automate most of the work involved in reducing chromatographic data. Even if I were not interested in working with macro languages, the native functionality of Excel would allow me to get through the day's chromatography a lot faster than struggling with Agilent's clunky old interface could, in all but the most routine matters. I have not seen the most recent (32 bit) version, and it may be that past experience is no longer a guide, but until I have, that's my two cents. Let me know if you want more information.

I assume/hope you got your problem sorted, but the simple answer would be: You're using a braketed standard run, therefore ChemStation won't generate any results till it has data from all the standards in each bracket.

Thanks all....I still can't solve my problem and I decided to use Ms Excel for my calibration plotting
Anyhow thanks