Getting started for HPLC method development

[viru]

I have very less knowledge about Method Development in HPLC, but i want to know how to start for mobile phase development and why we use buffers
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[WK]

Viru,
A direct answer to your question would be to read "Practical HPLC Method Development" by Snyder,Kirkland and Glajch (Wiley Interscience).

Can you not get some information on applications from companies that sell HPLC columns?
What is your application?
WK

[yaoguocan]

i quite agree with wk ,it is a best book for beginer .it is a book i always keep at hand,which i have read for many times.

[LarryHouse]

I usually start with a PubMed search on the drug you are looking do Method development on, find an appropriate journal article, and go from there...

[viru]

Thanks to all , I have seen that book, It's really good.

[Sallybeetle]

Besides PubMed, I also start with 1 - 3 of my favorite HPLC column manufacturers. I ask them for application notes using their columns and the compound(s) of interest.

[A.L.N. MURTHY]

Dear Sir,

We can help you by providing Applications Data on wide range of compounds FREE OF CHARGE .

You can contact me for any furthur help/ information.
regards,
A.L.N. MURTHY
CHIEF EXECUTIVE
CHEMINDIA
302, UBAS, BABUKHAN ESTATE,
BASHEERBAGH, HYDERABAD - 500 001.
TEL:+91-40-55904780/ 55904781
FAX: +91-40-27244169
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