Chondroitin Sulfate Content Analysis Method in a gel
Posted: Wed Mar 09, 2016 2:16 pm
by yildizliyim
Hi,
I researh a method about determination of chondroitin sulfate in gel form.
Do you have anybody who help me this subject?
BR
Re: Chondroitin Sulfate Content Analysis Method in a gel
Posted: Thu Mar 10, 2016 7:21 am
by Kreall
with HPLC method I woluld try with GPC. The right column for GPC is the key, you will have to know how big you are molecules in chondroitin polymer.
The gel form is always a pickle. Not a easy one.
As an alternative method you can always try titration with surfactant as a titrant (CPC or SDS), with phototrode from Mettler Toledo as a indicator of EQP. I do it on daily basis, as a quality control of drug with chondroitin sulphate. Unfortunatelly the drug from is power, so ca't help with the gel form.