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- Posts: 4
- Joined: Tue Oct 18, 2005 3:47 pm
what is carbon loading in HPLC and why is it important?
Most hplc is carried out by "reversed-phase" in which the stationary phase is essentially a layer of alkyl hydrocarbon bound to a silica gel substrate. If all other variables are held constant, a packing with a higher carbon load will have effectively a higher concentration of stationary phase and will provide greater retention.
In practice, other variables such as porosity, specific surface area, and bonding chemistry have a significant effect, an so carbon loadby itself does not really provide much useful information (in my opinion at least) .
