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polymeric reversed phase columns
Posted: Mon Oct 24, 2005 9:39 am
by bert
Hello!
Does anyone out there know a manufacturer of polymeric reversed phase columns (non-silica packing material). 0.3 mm ID??
thanks for your help,
regards Bert
Posted: Mon Oct 24, 2005 5:16 pm
by alealbor
Hi Bert,
You can found info about this kind of columns in:
http://www.polymerlabs.com/hplc/pharmacopeia.htm
Cheers
Alejandro
Posted: Tue Oct 25, 2005 1:17 am
by Uwe Neue
Supelco used to make a column like this. Have not checked on it recently.
Posted: Tue Oct 25, 2005 5:40 am
by tom jupille
I suspect the problem is less the "polymeric" than the "0.3 mm". I don't know of anyone who packs polymeric materials in that size column. You might check LC Packings (Dionex) for monolithic polymeric columns in that size range:
http://www1.dionex.com/en-us/columns_ac ... s5327.html
Posted: Wed Oct 26, 2005 6:22 am
by bert
Thanks for your help! I found what I needed
Regards Bert
Posted: Wed Oct 26, 2005 3:58 pm
by tom jupille
Okay, now share with the rest of us!

Posted: Fri Oct 28, 2005 5:44 am
by bert
Tom, lets share:
as Alejandro suggested, I contacted polymer labs and found exactly the column I wanted. I want to try this column for the teracycline analysis with the keto-enol tautomerism problem (previous thread). The idea is to use relative high salt concentrations in the mobile phase. I would like to see if excessive contamination of the source can be prevented due to the low flow I can use with the 300 µm column ......
Regards Bert
Posted: Fri Oct 28, 2005 5:49 pm
by tom jupille
Thanks, Bert!