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Running CSH column with 100% aqueous?

http://www.chromforum.org/viewtopic.php?t=27949

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Running CSH column with 100% aqueous?

Posted: Wed Jan 27, 2016 12:22 pm
by sulee154151
Can we run a Waters C18 CSH column with a 100% aqueous mobile phase without having any problem?

If not, what should be the minimum methanol or acetonitrile % in the mobile phase?

Thanks in advance.

Re: Running CSH column with 100% aqueous?

Posted: Thu Jan 28, 2016 12:51 am
by tom jupille
AFAIK, should be Ok with 100% water.

Re: Running CSH column with 100% aqueous?

Posted: Sun Feb 14, 2016 5:17 am
by mckrause
Unless your samples and standards are in 100% water you're not buying yourself anything with 100% mobile phase. I'd tend to run at least 5% organic phase and worry a lot more about the final solvent system of the sample.
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