Used distilled water instead of deionized water
Posted: Tue Dec 29, 2015 6:01 pm
by Saad89
Today Metrohm 930 compact IC Flux with conductivity detector was installed in our Laboratory and the technician accidentally used distilled water (Conductivity: 1.6 uS/cm) instead of deionized water as recommended in eluent, standard and regeneration solution preparation. The column was metrosep A 16-250/4.
Can this error damage the column and if yes is it revertible. Sorry for sounding like I have no idea as tomorrow will by my first day with the instrument
.
Re: Used distilled water instead of deionized water
Posted: Sun Jan 03, 2016 4:48 am
by M Farooq
Today Metrohm 930 compact IC Flux with conductivity detector was installed in our Laboratory and the technician accidentally used distilled water (Conductivity: 1.6 uS/cm) instead of deionized water as recommended in eluent, standard and regeneration solution preparation. The column was metrosep A 16-250/4.
Can this error damage the column and if yes is it revertible. Sorry for sounding like I have no idea as tomorrow will by my first day with the instrument
.
Distilled water will not damage the column but it is not as chemically pure as deionized water. Run a standard test chromatogram which comes when you purchase the column as a quality control test. If everything is well, the retention times will match the one provided by the manufacturer. If they are significantly off, contact the technician.
Re: Used distilled water instead of deionized water
Posted: Fri Jan 08, 2016 2:18 pm
by Markus Laeubli, Metrohm
Dear Saad89
M Farooq is absolutely right.
For further work I recommend to use ultrapure water (water purification system). Be aware the also bottled HPLC water is not recommended.
In case of any further question, do not hesitate to contact me.