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waters micromass triple quad is used for the quantification, why i always get quadratic regression from lcmsms analysis.
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quadratic regression
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Is this unexpected in the analysis you are doing? Are you operating near the lower sensitivity limit? How far off linear are your calibration curves?
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our lowest peak at LLOQ. linear regression give me r2 at 0.9.
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Ionization efficiency is often related to concentration if using Electrospray source. I often see quadratic responses on our LCMSMS.
The past is there to guide us into the future, not to dwell in.
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