-
- Posts: 1
- Joined: Sun Nov 22, 2015 4:00 am
Hi LC gurus,
May I get your advice on an baseline issue that I've never had with this Agilent 1260 LC? Even without actually doing a run, if I switch the mobile phase with mixed water and acetonitrile to 100% acetonitrile, after about 2 min once the pressure reached the lowest, the baseline would all of sudden increased and get stabilized. Then once I switch it back to the mixture of water and acetonitrile, again after 2 min once the pressure reached the highest, the baseline sharply decreased and stabilized there. I tried to change to fresh mobile phases, changed from 100% acetonitrile to 50% ACN/50% Methanol, changed to a different column. Still had the exactly same problem. Your suggestions would be greatly appreciated!
May I get your advice on an baseline issue that I've never had with this Agilent 1260 LC? Even without actually doing a run, if I switch the mobile phase with mixed water and acetonitrile to 100% acetonitrile, after about 2 min once the pressure reached the lowest, the baseline would all of sudden increased and get stabilized. Then once I switch it back to the mixture of water and acetonitrile, again after 2 min once the pressure reached the highest, the baseline sharply decreased and stabilized there. I tried to change to fresh mobile phases, changed from 100% acetonitrile to 50% ACN/50% Methanol, changed to a different column. Still had the exactly same problem. Your suggestions would be greatly appreciated!
