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HILIC particle: where can I find?
Posted: Wed Oct 21, 2015 11:35 am
by Humberto
Hello! =)
I want to make my own HILIC columns, since I am working with some samples that may destroy the almost 800€ commercial columns.
The problem is, I am having some issues to find just the particle for HILIC (the powder).
Someone could have any idea where to find? For now, could be any material, such silica, amino, amide, diol...
Thank you!
Humberto J.
Re: HILIC particle: where can I find?
Posted: Wed Oct 21, 2015 1:52 pm
by M Farooq
I want to make my own HILIC columns, since I am working with some samples that may destroy the almost 800€ commercial columns. The problem is, I am having some issues to find just the particle for HILIC (the powder). Someone could have any idea where to find? For now, could be any material, such silica, amino, amide, diol...Thank you!
Humberto J.
Daisogel and Kromasil sell silica particles. You need a column packing system to make your own columns (Knauer, and Haskel make such pumps).
Re: HILIC particle: where can I find?
Posted: Wed Oct 21, 2015 2:57 pm
by Andy Alpert
PolyLC has been selling the bulk packing material for HILIC ever since we introduced HILIC in 1990. It's called PolyHYDROXYETHYL A. This is silica with a very thick, polar coating of a neutral polypeptide covalently attached to silica. It's available in particle diameters from 3 to 20 micron and pore diameters from 60 to 1500 Å.
Re: HILIC particle: where can I find?
Posted: Wed Oct 21, 2015 3:13 pm
by Humberto
I want to make my own HILIC columns, since I am working with some samples that may destroy the almost 800€ commercial columns. The problem is, I am having some issues to find just the particle for HILIC (the powder). Someone could have any idea where to find? For now, could be any material, such silica, amino, amide, diol...Thank you!
Humberto J.
Daisogel and Kromasil sell silica particles. You need a column packing system to make your own columns (Knauer, and Haskel make such pumps).
Dear, Farooq!
Thank you for the suggestions!
I will get in contact with the companies!
Re: HILIC particle: where can I find?
Posted: Wed Oct 21, 2015 3:16 pm
by Humberto
PolyLC has been selling the bulk packing material for HILIC ever since we introduced HILIC in 1990. It's called PolyHYDROXYETHYL A. This is silica with a very thick, polar coating of a neutral polypeptide covalently attached to silica. It's available in particle diameters from 3 to 20 micron and pore diameters from 60 to 1500 Å.
Thank you for the suggestion, Herr Alpert!
I will shortly get in contact with you!