Hepatitis vaccine
Posted: Fri Oct 14, 2005 4:47 am
Hi. I'm trying to develop a RPLC method to quantify the hepatitis B surface antigen (HBsAg) in blood plasma but i don't know how to prepare the sample. I'm using the vaccine as the source of the HBsAg. For your information, the vaccine is a suspension (1ml) containing 20mcg of HBsAg, 500mcg aluminium hydroxide, 100mM phosphate buffer and 150mM sodium chloride.
From literature review, the HBsAg in yeast cell lysates were solubilized and reduced with a solution containing 4% SDS, 65% (v/v) mercaptoethanol and 1.3M DTT at 95 degree Celcius. But, since my HBsAg is in a suspension form, i don't whether the above sample prep is suitable. Also, is dilution of the vaccine possible and which diluent is recommeded?
Please assist me. Thank you.
From literature review, the HBsAg in yeast cell lysates were solubilized and reduced with a solution containing 4% SDS, 65% (v/v) mercaptoethanol and 1.3M DTT at 95 degree Celcius. But, since my HBsAg is in a suspension form, i don't whether the above sample prep is suitable. Also, is dilution of the vaccine possible and which diluent is recommeded?
Please assist me. Thank you.