Porapak Q performance test

[atiqah_ms]

Dear All.

I am newbie in this area.

I have a porapak Q 80/100 chromosob packing. the column was not use for several if years (according to my senior it has been kept in a box for about 10 years). They also did not know the condition of the column since the column was purchase from second buyer (may be I am the 2nd or 3rd user).

I have inject ethanol through it with the program setting as below:
Injection temp: 150, detector: 200 and oven: 120
attenuation:16
N2 flow rate: 30ml/min
Ethanol peak may be expected at RT: 1.5

Unluckily, no pear appear

By the way, I am using PE autosytem with FID

Can someone share on the basic test using this column?
(specified the setting and condition)

Hope to hear some feedback.


Thanks
Sofia
Malaysia

[chromatographer1]

First, confirm you have flow through the column. If it is a 2.1mm ID column set your carrier flow at 30cc/min If 4mm, at 60cc/min.

Then set the temperature of the oven at 180C and the injector and detector at 200C.

Inject methanol ethanol isopropanol in an aqueous solution of 10mg/mL. Inject 1 microliter of solution.

You should have 3 clearly separated peaks with little tailing (tf < 1.3)

You can also add acetone to the solution if you wish.

best wishes,

Rod

[HPHealth]

Dear Sofia,

Any news about your issue. Is chromatrographer1 idea worked?

Thanks,