Glimepiride Isomers

[syx]

Dear Members,
Does anyone have experience with separation method for glimepiride isomers based on J. Sep. Sci. 2003, 26, 1595-1597?
Here are the chromatographic conditions:
Column: Dikmonsil C18 (250 x 4.6mm, 5um)
Mobile phase: MeOH : ACN : HAc-NH4Ac (1.5 mol/L, pH 4.5) = 1.1:1.3:1.0 (v/v)
Flow rate: 0.5 mL/min
UV detection: 228 nm
Injection volume: 10 uL
Sample concentration: 100 ug/mL (MeOH as the solvent)
When we tried to adopt the method (using different brand of column) we have got trouble: baseline error. It may be due to very high salt concentration in the mobile phase. Acetate gives absorbance in used wavelength area, especially in such concentration.

For everyone who had use this method and got good result, please let me know how.
Or are there other references for glimepiride isomers separation?

Best regards,
Siswanto Tanuatmojo

[Mark Tracy]

You are right. The acetate concentration is surprisingly high. Can you communicate with the authors of that article? Perhaps they can explain their choice of buffer; or perhaps there is an error in the article.

[amaryl]

Dear Members,
Does anyone have experience with separation method for glimepiride isomers based on J. Sep. Sci. 2003, 26, 1595-1597?
Here are the chromatographic conditions:
Column: Dikmonsil C18 (250 x 4.6mm, 5um)
Mobile phase: MeOH : ACN : HAc-NH4Ac (1.5 mol/L, pH 4.5) = 1.1:1.3:1.0 (v/v)
Flow rate: 0.5 mL/min
UV detection: 228 nm
Injection volume: 10 uL
Sample concentration: 100 ug/mL (MeOH as the solvent)
When we tried to adopt the method (using different brand of column) we have got trouble: baseline error. It may be due to very high salt concentration in the mobile phase. Acetate gives absorbance in used wavelength area, especially in such concentration.

For everyone who had use this method and got good result, please let me know how.
Or are there other references for glimepiride isomers separation?

Best regards,
Siswanto Tanuatmojo

hey siswanto

well did you use cis isomer of glime in your analysis. apart from baseline error did u get separation of cis and trans isomer.


well the article specifies the same chromatographic conditions.

regards

Amaryl

[syx]

Still could not be separated. I do not have the column as specified in the journal, so I use different brand. I have found 3 methods for the separation: 2 using acetate in the wavelength of below 240 nm (result in high baseline disturbance) and 1 using L20 column packing.
I have tried to contact the correspondent person in the journals, but still no reply.

[amaryl]

Still could not be separated. I do not have the column as specified in the journal, so I use different brand. I have found 3 methods for the separation: 2 using acetate in the wavelength of below 240 nm (result in high baseline disturbance) and 1 using L20 column packing.
I have tried to contact the correspondent person in the journals, but still no reply.

could you please send me the links to other two methods if possible.

thanking you

regards

Amaryl

[syx]

I sent it to your mail...