Derivatisation of protein for SEC
Posted: Thu Sep 10, 2015 10:43 am
Hi,
We have a sixe-exclusion method that separates protein from protein aggregates. The problem is that polysorbate in the sample gives interferences, so we are not sure what is aggregates and what is polysorbate (probably present as micelles).
Would it be possible to label the protein + aggregates with a fluorescent dye before analysis, and then run SEC/Fluorescence instead of SEC/UV?
If you have an idea of dye and protocol, please give me your suggestion!
thanks
We have a sixe-exclusion method that separates protein from protein aggregates. The problem is that polysorbate in the sample gives interferences, so we are not sure what is aggregates and what is polysorbate (probably present as micelles).
Would it be possible to label the protein + aggregates with a fluorescent dye before analysis, and then run SEC/Fluorescence instead of SEC/UV?
If you have an idea of dye and protocol, please give me your suggestion!
thanks