Problem with ELSD detector
Posted: Sun Aug 30, 2015 12:24 pm
by sokzkaralucha
Hello everyone, I work with ELSD detector recently (conjugate with HPLC system), unfortunetly I don't have enought experience. I've one problem during the work. I seperate two substances, that shoud have two another signal. Unfortunelty, I see two double signals with low resolution and I don't know why. Substances are stable, one of them I can see using UV detector. I use Sedex ELSD with flow 1,0 ml/min. Thanks for any ideas (;
Re: Problem with ELSD detector
Posted: Tue Sep 01, 2015 5:49 am
by kubowicz.tomasz
Hello
What do you mean by "double signals"?
I seperate two substances, that shoud have two another signal. Unfortunelty, I see two double signals with low resolution and I don't know why. (;
Do you mean peaks?
If you expect quick and clear answer you should describe problem with more details...Please send information about:
-method (parameters)
-detectors (parameters)
-paste chromatogram with "problematic" run
Otherwise it is like swimming in the ocean...
Regards
Tomasz Kubowicz