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Problem with ELSD detector
Discussions about HPLC, CE, TLC, SFC, and other "liquid phase" separation techniques.
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Hello everyone, I work with ELSD detector recently (conjugate with HPLC system), unfortunetly I don't have enought experience. I've one problem during the work. I seperate two substances, that shoud have two another signal. Unfortunelty, I see two double signals with low resolution and I don't know why. Substances are stable, one of them I can see using UV detector. I use Sedex ELSD with flow 1,0 ml/min. Thanks for any ideas (;
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Hello
What do you mean by "double signals"?
If you expect quick and clear answer you should describe problem with more details...Please send information about:
-method (parameters)
-detectors (parameters)
-paste chromatogram with "problematic" run
Otherwise it is like swimming in the ocean...
Regards
Tomasz Kubowicz
What do you mean by "double signals"?
Do you mean peaks?I seperate two substances, that shoud have two another signal. Unfortunelty, I see two double signals with low resolution and I don't know why. (;
If you expect quick and clear answer you should describe problem with more details...Please send information about:
-method (parameters)
-detectors (parameters)
-paste chromatogram with "problematic" run
Otherwise it is like swimming in the ocean...
Regards
Tomasz Kubowicz
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