Backpressure Calculation

[Johte]

Hello all,

I am trying to pack pulled tip nano columns with Tosoh TSKgel Amide-80 5 um material recovered from a column. In my first attempt I either crushed the particles in packing or online with too high of a pressure. It ended up running like size exclusion with the same profile regardless of gradient.

The most information I can find is the back pressure limits for different sized columns. What equation can I use to calculate the amount of back pressure on my nano column based on the commercial? I figure it has to involve the amount of force applied to the material but I'm unsure of how to compensate for the diameter.

Thanks

[tom jupille]

Other things being equal, the back pressure will vary inversely with the square of the diameter.