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SEPARATION PROBLEM

http://www.chromforum.org/viewtopic.php?t=27221

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SEPARATION PROBLEM

Posted: Mon Aug 10, 2015 6:36 pm
by MAdesok
Hi everyone,
I am using RP C18 column from Waters and analyzing carotenoids. Kindly assist on how to rectify error message such as bubbles in the compression and sometimes the pressure suddenly rise excessively. Please advise on causes of bubbles and how to rectify it on my system as well as pressure fluctuations. I operate with a flow rate of 1 ml/per minute with a PDA detector.

Thank you

Re: SEPARATION PROBLEM

Posted: Tue Aug 11, 2015 3:53 pm
by tom jupille
the first and most obvious think to look at is whether your degasser is operating properly. If this is an isocratic separation, you might consider premixing and then degassing your mobile phase off-line.

Re: SEPARATION PROBLEM

Posted: Wed Aug 12, 2015 6:48 am
by MAdesok
Thank you for your input.

Re: SEPARATION PROBLEM

Posted: Thu Aug 20, 2015 10:20 am
by danko
The pressure rise is caused by something else.
Is it systematic - f.x. upon injection?
And what happens NeXT - does the pressure normalise?

Best Regards

Re: SEPARATION PROBLEM

Posted: Thu Sep 10, 2015 3:50 pm
by HippyLabRat
What is/are your mobile phase(s)? Flow Rates?
Can't help diagnose without this problem.
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