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Drift and noise on HPLC analysis
Posted: Mon Aug 03, 2015 1:57 pm
by Hawine
Hi
I was analysing sugar in honey on HPLC RI detector. I got good results previously when I start the analysis but after some time I faced a problem on the baseline drift and noise so I couldn't integrate the peaks. Do you have any Sugestion please. I spent more than a week by backflushing the column with Isopropanol but the problem was not solved.
Re: Drift and noise on HPLC analysis
Posted: Tue Aug 04, 2015 6:44 am
by Gerhard Kratz
A little bit more information would be nice. Do you use a NH3 column? You have A) a temperature problem and B) a degassing problem. Your column is not the problem. Before start flush your reference cell with fresh MP. Good luck
Re: Drift and noise on HPLC analysis
Posted: Tue Aug 04, 2015 9:28 am
by mattmullaney
Hi Hawine,
More info is always better...seems a good week for RI troubles. Here's a link to look at, Gerhard's very likely on to what the trouble is:
http://www.chromatographyonline.com/avo ... problems-0
Temperature and mobile phase equilibration are usual suspects with RI detection. Best Wishes!
Re: Drift and noise on HPLC analysis
Posted: Tue Aug 04, 2015 1:51 pm
by Hawine
Yes Gerhard Kratz I'm using NH2 Column size: 4.6x250mm, 5 micron and thank you for your reply I'm trying the suggesion you gave me.
Re: Drift and noise on HPLC analysis
Posted: Thu Aug 06, 2015 7:08 am
by Hawine
Thank you all for the link you share for me and the advice you gave me it helped a lot. The system become very stable I'm able to do my analysis now.