Drift and noise on HPLC analysis

[Hawine]

Hi
I was analysing sugar in honey on HPLC RI detector. I got good results previously when I start the analysis but after some time I faced a problem on the baseline drift and noise so I couldn't integrate the peaks. Do you have any Sugestion please. I spent more than a week by backflushing the column with Isopropanol but the problem was not solved.

[Gerhard Kratz]

A little bit more information would be nice. Do you use a NH3 column? You have A) a temperature problem and B) a degassing problem. Your column is not the problem. Before start flush your reference cell with fresh MP. Good luck

[mattmullaney]

Hi Hawine,

More info is always better...seems a good week for RI troubles. Here's a link to look at, Gerhard's very likely on to what the trouble is:

http://www.chromatographyonline.com/avo ... problems-0

Temperature and mobile phase equilibration are usual suspects with RI detection. Best Wishes!

[Hawine]

Yes Gerhard Kratz I'm using NH2 Column size: 4.6x250mm, 5 micron and thank you for your reply I'm trying the suggesion you gave me.

[Hawine]

Thank you all for the link you share for me and the advice you gave me it helped a lot. The system become very stable I'm able to do my analysis now.