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RP-LC/MS Standard

http://www.chromforum.org/viewtopic.php?t=27167

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RP-LC/MS Standard

Posted: Thu Jul 30, 2015 6:18 pm
by Johte
Hello all,

I'm running Ammonium acetate/Acetonitrile gradients on 75 um C18 columns that I pack. I spray specifically in negative mode for nucleic acids so I'd like to have something larger in the mix.

I was hoping someone had some suggestions of what compounds I could use as the typical RP standards are not ideal for negative mode. ( Uracil, caffeine, etc)

Much obliged!

Re: RP-LC/MS Standard

Posted: Fri Aug 14, 2015 9:07 pm
by kmchale
Johte,

I regularly use the antibiotic Chloramphenicol to evaluate LC/MS systems in negative ESI mode.
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