Chromatography Forum

Hey All

Can anyone point me towards any commercial columns that attach directly to the injector and detector.

In other words, most LC columns are connected by virtue of fittings and tubing. The only exception I can think of is one company that sells polymeric monoliths, where the column itself attaches directly to the injector and detector: and I guess it is the narrowness of the column that makes this possible. It seems to me that such a thing might be possible with, for example, standard 2 mm ID columns. But I don't think I've seen this.

Can anyone point me towards any such commercial products.

Thanks for your assistance!!

I'd think columns could be connected to some detectors using a 2-way column connector union, male threads on each end.

But then they wouldn't be heat-controlled, temperature may vary with the room.

Hey All

Can anyone point me towards any commercial columns that attach directly to the injector and detector.

In other words, most LC columns are connected by virtue of fittings and tubing. The only exception I can think of is one company that sells polymeric monoliths, where the column itself attaches directly to the injector and detector: and I guess it is the narrowness of the column that makes this possible. It seems to me that such a thing might be possible with, for example, standard 2 mm ID columns. But I don't think I've seen this.

Can anyone point me towards any such commercial products.

Thanks for your assistance!!

Could you explain the purpose of such a set up. Are you trying to eliminate extra-column volume? A typical capillary column can somewhat fulfill your requirements.

A very good idea to avoid extra column effects and connection problems.
Unfortunately not practicable. We need to use different column lengths and column heaters. On one end the whole system must be flexible to adjust different column lengths. A good training to make dead volume free connections would also help. Make all tubing from injector to column and from column to detector as short as possible. I learned to cut a stainless steel tubing even with a Swiss army knife! It works.

I learned to cut a stainless steel tubing even with a Swiss army knife! It works.

Can you post this as a video tutorial? That would be REALLY helpful.

Thanks
Jörg

Hello

Could you please give us a reason why you want minimize extra volume with so drastic solution (connect column directly to injector and detector)?
The best way of course is to keep capillary connections as short as you can but there is a point where trying make it shorter is pointless because impact on chromatography is negligible.

Best regards

Tomasz Kubowicz

Hi Jörg,

MAybe later such a video can be done.
Just do a scratch on the tubing with your knife, than move the tubing from both ends up and down and than you need to get a smooth surface using a file.
I had always a magnifier with me, a small one to check the crack and if the inner diameter is good.
Before I used the new tubing I connected it to a syringe and flushed it with ACN.

we have a version of hardware with one mail/one female end, similar to our guards:
http://www.sielc.com/Products_GuardColu ... mesep.html