Chromeleon 6.8 / Multiple channel calibration

[ATHERRIEN]

I want to integrate and report peaks from multiple different channels. All the help files I have found concern multiple peaks from one channel.

I am acquiring some 25 SIM channels corresponding to 25 compounds and I would like to produce 1 calibration curve per channel. At the moment I have to integrate and do the 25 calibrations manually on excel which is very tedious.

I tried to automate the integration via the QNT-editor, but it seems that the "autogenerate peak table" only integrates the peaks from the active channel, not all of them.

Any ideas? Thank you.

[ATHERRIEN]

What I think I am missing is how to simply tell chromeleon that "Peaks in channel x are compound x". I don't need retention time at all, and technically I don't even need mass. Removing all criteria and adding an exact mass filter with a specific channel like this does not work:



The program seems to really only assign peaks based on time, and then check for mass. This fails for me in part because I am using isotopes.

Everything is fine when I manually assign the peaks:



But if I don't, peaks are not recognized:



Thank you!

[sbrand]

Hi Atherrien

There is maybe solution in Chromeleon. Open the qnt and right click in the column "Ret Time" of the peak table. Then choose "columns" and "duplicate column". Select the channel for this Ret Time. Therefore you can create a channel selective component table and calibration. Does this solve you problem?

Regards and success

[ATHERRIEN]

Hi sbrand,

I had forgotten about this thread until google brought me here again. I just tested your solution and I think it works!

Many thanks