-
- Posts: 1
- Joined: Tue Jun 26, 2012 8:20 pm
Advertisement
get phenyl propionate into H2SO4 mobile phase
Discussions about HPLC, CE, TLC, SFC, and other "liquid phase" separation techniques.
2 posts
Page 1 of 1
I will be running biological waste samples by HPLC and need to develop a standard curve for potential analytes. The SOP is using a Rezex ROA-Organic acid H+ column from phenomenex with 0.005M H2SO4 mobile phase. One analyte is phenyl propionate in a range of .025% to 1%. I am having an issue with getting the phenyl propionate standard into a 0.005M H2SO4 solution. I have tried heating and alcohol carriers but have not been successful. Initially I didn't want to use too high of a concentration of a carrier so that it would interfere with the run however I am willing to try anything. If anyone has any experience with this I would very much appreciate any suggestions. Thank you.
-
- Posts: 715
- Joined: Wed Aug 04, 2010 2:45 pm
I would also try a micro wave to get it into solution.
Good luck
Good luck
Gerhard Kratz, Kratz_Gerhard@web.de
2 posts
Page 1 of 1
Who is online
In total there are 16 users online :: 0 registered, 0 hidden and 16 guests (based on users active over the past 5 minutes)
Most users ever online was 4374 on Fri Oct 03, 2025 12:41 am
Users browsing this forum: No registered users and 16 guests
Most users ever online was 4374 on Fri Oct 03, 2025 12:41 am
Users browsing this forum: No registered users and 16 guests
Latest Blog Posts from Separation Science
Separation Science offers free learning from the experts covering methods, applications, webinars, eSeminars, videos, tutorials for users of liquid chromatography, gas chromatography, mass spectrometry, sample preparation and related analytical techniques.
Subscribe to our eNewsletter with daily, weekly or monthly updates: Food & Beverage, Environmental, (Bio)Pharmaceutical, Bioclinical, Liquid Chromatography, Gas Chromatography and Mass Spectrometry.
- Follow us on Twitter: @Sep_Science
- Follow us on Linkedin: Separation Science
