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HPLC method for ascorbic acid determination in a cream drug
Posted: Tue Jul 07, 2015 11:49 am
by gnikol21
Hi all
I want to dermine the content of ascorbic acid in a cream drug product. Can anyone share any HPLC method for doing this?
Thnx in advance
Re: HPLC method for ascorbic acid determination in a cream d
Posted: Tue Jul 07, 2015 2:48 pm
by Consumer Products Guy
Do you have refractive index detector there? Conductivity detector? ELSD?
My mind-reading powers have long vanished....
Re: HPLC method for ascorbic acid determination in a cream d
Posted: Tue Jul 07, 2015 2:59 pm
by gnikol21
I have an HPLC with RI detector.
Re: HPLC method for ascorbic acid determination in a cream d
Posted: Tue Jul 07, 2015 5:33 pm
by Consumer Products Guy
I have an HPLC with RI detector.
OK. Now let us know if you're looking in the 100 ppm range, 1000 ppm range, 1% range, etc.
Re: HPLC method for ascorbic acid determination in a cream d
Posted: Wed Jul 08, 2015 9:23 am
by Gerhard Kratz
Cream means fat?? In that case, during sample preparation do ultra sonic and than store your sample in the freezer for 15 minutes, than centrifugate and it should be a clear liquid for injection.
Check application books of different manufacturers of HPLC columns. Good luck
Re: HPLC method for ascorbic acid determination in a cream d
Posted: Wed Jul 08, 2015 11:36 am
by gnikol21
Thnx Gerhard for your help i will try this extraction to check the recovery and i hope to be fast enough to prevent the degradation of ascorbic acid
Re: HPLC method for ascorbic acid determination in a cream d
Posted: Wed Jul 08, 2015 11:37 am
by gnikol21
well Im working in a range around 50ppm
Re: HPLC method for ascorbic acid determination in a cream d
Posted: Wed Aug 19, 2015 9:44 pm
by HippyLabRat
For extraction solution/sample diluent I used 1% meta-phosphoric acid (HPO3 not H3PO4) in water. Sigma was the least expensive supplier I could find. I was using this for extracting multivitamins, and I was getting preservation on the order of about 5 days with about a 2% loss in concentration over that time.
Hope this helps.