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Analysis of amines by HPLC
Posted: Sat Jun 27, 2015 3:30 pm
by Oatsandbeans
I am trying to spec out a HPLC instrument. I need to do analysis of primary alpihatic amines ( amongst other things which are aromatic). Will a variable wavelength detector be able to handle this ? The levels of the amines will be high so I dont't need great sensitivity. There is lots of papers about derivatization for amine analysis which seems like something I want to avoid if possible. Any input would be really appreciated.
Re: Analysis of amines by HPLC
Posted: Sat Jun 27, 2015 8:12 pm
by tom jupille
Aliphatic amines do not have much (i.e. any) absorbance. Refractive index would be a better bet, especially if your levels are high. Problems with RI, of course, are that it will respond to *everything* in the sample, it's incompatible with gradients, and temperature control is critical.
Re: Analysis of amines by HPLC
Posted: Sun Jun 28, 2015 8:41 am
by Oatsandbeans
Tom,
Thanks, I suspected that aliphatic amines might not work well with a UV detector. It is difficult because lots of my materials are aromatic that will be great on UV. I may see if I can squeeze 2 detectors out of the budget a UV and a RI.
Re: Analysis of amines by HPLC
Posted: Tue Jun 30, 2015 9:51 pm
by Vlad Orlovsky
Re: Analysis of amines by HPLC
Posted: Wed Jul 01, 2015 1:23 pm
by Oatsandbeans
Vlad,
thanks for that.