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method validation

http://www.chromforum.org/viewtopic.php?t=26988

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method validation

Posted: Sat Jun 20, 2015 9:59 am
by mritunjai078
Dear Friends,

We have validated method in which sample dilution is 100mg/100ml and further 10ml/100ml (100ppm solution).
Can we change the sample preparation to 25mg/250ml (100 ppm) to avoid pipetting error.
If yes, we have to revalidate the method or not!

Regards

Re: method validation

Posted: Sat Jun 20, 2015 2:13 pm
by Peter Apps
There are two issues with reducing sample quantity - the relative uncertainty in sample mass will rise (unless you change your weighing procedure as well) and sample inhomogeneity will become more of a problem. These might cancel any improvement due to omitting the dilution step.

Peter
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